What is recommended as a remedy if frozen tissue crumbles during cutting?

When frozen tissue crumbles during cutting, refreezing the tissue block is an appropriate remedy. This approach addresses the issue of tissue integrity. When tissue is too warm or not sufficiently frozen, it can lose its structure and become crumbly, making it difficult to obtain clean sections. By refreezing the tissue, the goal is to restore rigidity, allowing for more successful and uniform cuts. This can ensure that the cellular architecture is preserved for accurate histopathological analysis.

The other options, while they may seem plausible, are less effective for the specific issue of crumbly frozen tissue. Allowing the tissue to thaw would likely worsen the crumbling. Changing the blade might help in cases where the blade is dull, but it doesn't remediate the underlying problem of the tissue's condition. Soaking the tissue in a staining solution would be inappropriate, as it would not address the crumbling and could potentially further damage the sample. Thus, refreezing the specimen remains the most effective and direct solution to maintaining a quality sample for microscopic examination.