What is the optimal temperature setting for a water bath when floating out cut sections?

The optimal temperature setting for a water bath when floating out cut sections is in the range of 45-50 degrees Celsius. This temperature is ideal because it is close to the melting point of paraffin wax, which generally ranges from around 55 to 65 degrees Celsius. When sections are floated on a water bath at this temperature, the wax begins to become pliable and allows for easier manipulation and positioning of the sections for staining.

Using temperatures that are too low, such as below 10 degrees Celsius of the melting point of wax, would not adequately soften the wax. This could result in difficulties during the floating of the sections, leading to tearing or distortion. Additionally, temperatures that are too high, such as above 60 degrees Celsius, may damage the tissue samples or cause the wax to become overly fluid, compromising the integrity of the sections. Thus, the optimal temperature ensures that the wax is sufficiently softened while maintaining the structure of the tissue being prepared.