What is used with osmic acid to fix and stain blood and glandular tissues?

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The appropriate choice for fixing and staining blood and glandular tissues in conjunction with osmic acid is the use of Rhodamine B. Osmic acid is primarily used as a fixative due to its ability to preserve lipids and provide excellent morphological detail. However, it is not inherently a staining agent. Rhodamine B serves as a fluorescent stain that can interact well with various cellular components, particularly proteins and other cellular structures. When paired with osmic acid, Rhodamine B enhances the visualization of tissues by imparting fluorescence, thereby allowing for more detailed examination of cellular components in the blood and glandular tissues. The synergistic effect of these two agents makes them effective for this specific application in histopathology.

Other options may be effective for different purposes but are not specifically used alongside osmic acid for the fixation and staining of blood and glandular tissues.

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