What stain is used to detect alkaline phosphatase (ALP)?

The Gomori Calcium stain is specifically designed to detect alkaline phosphatase (ALP) activity in tissues. This enzyme is often associated with processes involving calcium and phosphate metabolism, which is why a stain that highlights calcium deposits can indirectly indicate the presence of ALP activity. The staining technique essentially visualizes areas of tissue where alkaline phosphatase is active, allowing pathologists to assess conditions related to bone metabolism or certain types of tumors.

Other stains mentioned, while useful for various histopathological applications—such as Gomori Lead, Krajian's Amyloid stain, and Mallory's stain—do not specifically target alkaline phosphatase or its related enzymatic activity. Instead, they are more suited for identifying lead deposits, amyloid proteins, and liver pathology, respectively. This specificity makes Gomori Calcium stain the appropriate choice for detecting alkaline phosphatase activity in tissues.