Which method is used to demonstrate glycogen with a mahogany brown color?

The Langhan's iodine method is specifically designed to demonstrate glycogen within tissue samples, producing a distinct mahogany brown color as a result of the interaction between iodine and glycogen. When iodine binds to glycogen, it forms a complex that imparts this characteristic coloration, making it easier to identify and study glycogen deposits in histological sections.

In contrast, the Feulgen method is used to stain DNA, resulting in a magenta coloration, which is not applicable for glycogen detection. Osmic acid stain is primarily used for staining lipids and has no relevance to glycogen. Hematoxylin staining is utilized for visualizing nuclei and other cellular structures but does not produce a specific reaction with glycogen either.

Thus, the Langhan's iodine method is the established technique for glycogen demonstration, underscoring its importance in histopathology for visualizing this carbohydrate within tissues.